Computational Survey Identifies 35 WRKY Transcription Factor Genes in Chia
Protein lengths ranged from 143 amino acids for CsWRKY21 to 606 amino acids for CsWRKY20, with a mean of 332.8 amino acids. The mean molecular weight was 37,499 daltons and the mean theoretical isoelectric point was 7.62, spanning a range from 5.16 to 9.67. All proteins were predicted to be hydrophilic, with uniformly negative GRAVY values and a mean aliphatic index of 76.25. The study's limitations are stated with unusual candor. No wet-laboratory experiments were performed to validate gene identity, expression patterns, protein-DNA interactions or protein-ligand binding. All analyses rest on a single reference genome assembly, so cultivar-level genetic variation in chia was not captured. What the study delivers, therefore, is a preliminary but coherent genomic framework: 35 named and classified WRKY genes, their chromosomal addresses, duplication history, promoter architecture…
