Alfalfa tissue-culture platform speeds regeneration and elite-cultivar transformation
An integrated alfalfa tissue-culture system produced faster regeneration and transformation, with a 2.8-fold increase in transgene-positive plants and complete regeneration in all 25 cultivars tested. A stage-integrated tissue-culture platform developed for elite alfalfa increased the frequency of transgene-positive plants 2.8-fold and shortened the transformation cycle by two to three months compared with a previously reported protocol The system was developed primarily with the elite cultivar âZhongmu No 1,â which has a high-quality reference genome, and combines axenic clonal explants, low-browning embryogenic callus induction, somatic embryo development without plant growth regulators, and direct plantlet conversion Its broader regeneration response was demonstrated in all 25 cultivars tested, although the reported cross-cultivar result concerned regeneration rather than…
