A new protocol details PINE-TREE, a transient fluorescent reporter system that enriches prime-edited human pluripotent stem cells and yields up to 80 percent editing efficiency in sorted populations.
A new closed-tube RT-RAA-CRISPR/Cas12a assay detects Schmallenberg virus within 50 minutes and outperformed RT-qPCR on simulated bovine nasal swab samples, though validation with authentic clinical specimens is still pending. Subject of Research: Development of a rapid visual RT-RAA-CRISPR/Cas12a nucleic acid assay…
Genes contain instructions for making proteins, and a central dogma of biology is that this information flows from DNA to RNA to proteins. But only two percent of the human genome actually encodes proteins; the function of the remaining 98 percent…
What doesn’t kill a bacterium makes it stronger. An enzyme that bacteria use to fight off viruses works by targeting not just the virus, but also the bacterium itself. It sends the bacterium into a dormant state and makes it…
A new review highlights the potential of CRISPR–Cas13 for RNA-editing therapies. Discussing the advantages of the effector protein Cas13 over Cas9, they report that CRISPR–Cas13 facilitates RNA knockdown, RNA silencing, RNA splicing, RNA-base editing, and translation regulation. CRISPR–Cas13 has diverse applications like…
A new review argues that CRISPR antivirals, especially RNA-targeting Cas13 systems, can suppress respiratory viruses in preclinical models, with lung delivery, safety, and regulation now the main barriers to clinical use. News Source: Juliet Wilcox. (October 29, 2026). CRISPR Antivirals Target Respiratory Viruses,…
For much of human history, animals and plants were perceived to follow a different set of rules than the rest of the universe. In the 18th and 19th centuries, this culminated in a belief that living organisms were infused by a non-physical…
Researchers identified a site upstream of the PEDV M gene that supports stable expression of FMDV VP1 in a recombinant porcine epidemic diarrhea virus without impairing replication in cells or piglets. Subject of Research: Identification of a genomic insertion site upstream…
An engineered compact Cas12m variant enables multiplexed CRISPR gene activation from a single AAV vector in mouse models. Researchers have engineered a compact CRISPR-based system that can switch on multiple genes at once in living animals, packaged within a single adeno-associated…
Researchers in Wuhan, China, have developed a rapid diagnostic assay that detects armA, a gene that confers high-level resistance to aminoglycoside antibiotics in bacterial pathogens. The method combines multiple cross displacement amplification (MCDA) with the CRISPR/Cas12a system, and the team…
New gene editors and how they work: Cas enzymes, base and prime editing, epigenetic editors, recombinases and ways of delivering editors into cells.
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